Electrophoresis of normal RNA can be done in a 2% agarose gel in about 1 h while, Ribosomal RNAs (18 S and 28 S) are clearly resolved in 2.5–5% acrylamide gradient gel with an overnight run. 

TLS Online TPP Program

#Id: 601

#Unit 2. Cellular Organization

TLS Online TPP Program

#Id: 602

#Unit 2. Cellular Organization

CII is a very unstable protein in E. coli; it is degraded by a specific protease called FtsH (HflB), encoded by the hfl gene. 

TLS Online TPP Program

#Id: 603

#Unit 2. Cellular Organization

Cells lacking the hfl gene (and thus FtsH) almost always form lysogens upon infection by lambda Then What is fate of cI-?

TLS Online TPP Program

#Id: 604

#Unit 2. Cellular Organization

The N gene codes for an antitermination factor whose action at nut (N utilization) sites allows transcription to proceed into the delayed early genes

 

TLS Online TPP Program

#Id: 605

#Unit 2. Cellular Organization

N protein prevents termination in the early operons of lambda and it works together with the products of the bacterial genes nusA, nusB, nusE, and nusG.

TLS Online TPP Program

#Id: 606

#Unit 2. Cellular Organization

N binds to RNA transcribed from DNA containing a nut sequence. Thus, once RNA polymerase has passed a nut site, N binds to the RNA and from there is loaded on to the polymerase itself. In this state, the polymerase is resistant to the terminators found just beyond the N and cro genes.