Electrophoresis of normal RNA can be done in a 2% agarose gel in about 1 h while, Ribosomal RNAs (18 S and 28 S) are clearly resolved in 2.5–5% acrylamide gradient gel with an overnight run. 

#Id: 2497

#Unit 13. Methods in Biology

In the Native (Buffer) Gels electrophoresis it not possible to predict the behavior of a given protein in a buffer gel, but, because of the range of different charges and sizes of proteins in a given protein mixture, good resolution is achieved.

#Id: 2498

#Unit 13. Methods in Biology

Gradient Gels electrophoresis are performed using gradient of  acrylamide Concentration that varies uniformly from, typically, 5% at the top of the gel to 25% acrylamide at the bottom of the gel.

#Id: 2499

#Unit 13. Methods in Biology

There are two major advantages to running gradient gels. 

First, a much greater range of protein Mr values can be separated than on a fixed- percentage gel.

#Id: 2500

#Unit 13. Methods in Biology

The second advantage of gradient gels is that proteins with very similar M r values may be resolved.

#Id: 2501

#Unit 13. Methods in Biology

The higher no. of band will be observed in gradient gel electrophoresis comparison to uniform gel SDS and Native gel electrophoresis.

#Id: 2502

#Unit 13. Methods in Biology

Isoelectric Focusing Gels is based on the separation of molecules according to their different isoelectric points at which the show zero electrophoretic mobility.