Proximal tubular reabsorption

Reabsorbs 67% of the filtered Na+

Reabsorbs 67% of the filtered water

Reabsorbs 100% of the filtered glucose & amino acids 

Reabsorbs 85% of the filtered HCO3−

Reabsorbs most of the filtered phosphate, lactate, and citrate

TLS Online TPP Program

#Id: 919

#Unit 13. Methods in Biology

Other dyes that bind to DNA and allow for similar types of cell cycle analysis include DAPI, Hoechst 33342, and 7-aminoactinomycin D (AAD).

TLS Online TPP Program

#Id: 920

#Unit 13. Methods in Biology

Carboxyfluorescein succinimidyl ester (CFSE) is more correctly named carboxyfluorescein diacetate succinimidyl ester (CFDASE) very commonly used to detect state of cell 

TLS Online TPP Program

#Id: 921

#Unit 13. Methods in Biology

(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles

TLS Online TPP Program

#Id: 922

#Unit 13. Methods in Biology

CFSE labeling amount of fluorescence emitted is cut in half each time the cell divides.

TLS Online TPP Program

#Id: 923

#Unit 13. Methods in Biology

The 51Cr Release Assay is used to measure Cell death not apoptosis

TLS Online TPP Program

#Id: 924

#Unit 13. Methods in Biology

Normal cells treated with sodium chromate allowed to absorb and induced to death signals as T-CTLs Death of the target cells is indicated by the release of 51Cr into the supernatant