In case of nested PCR, the inner primers have a lower annealing temperature than that of the outer pair. For the initial several cycles of PCR the higher denaturation and annealing temperatures are used; as a result, only the outer pair of primer is able to prime amplification. 

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#Id: 8662

#Unit 10. Ecological Principles

Coexist of species 

They Do Not Occupy Identical Niches

Species may partition resources

Morphological differences

Realized niche is less than fundamental niche 

Greater strength intraspecific competition than interspecific competition 

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#Id: 4657

#Unit 13. Methods in Biology

(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles

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#Id: 8918

#Unit 2. Cellular Organization

Centralspindlin is a heterotetramer composed of two copies of the kinesin-6 motor protein MKLP1 and a dimer of a Rho GAP

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#Id: 2267

#Unit 11. Evolution and Behavior

Breeder’s equation, predict how much evolutionary change will result from selective breeding.

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#Id: 6005

#Unit 3. Fundamental Processes

Orc2-5 binds strongly; Orc6 binds weakly to ARS and has a nuclear localization signal that must be activated by the cyclin/ CDK kinase during the G1 to S transition.