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Touchdown  PCR is initiated at very high annealing temperatures, which allow only perfectly matched primer-template DNA hybrids to form and support amplification. The annealing temperature is dropped in a stepwise fashion with each cycle of PCR. 




#Unit 13. Methods in Biology #PCR #Part B Pointers
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TLS Online TPP Program

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#Unit 6. System Physiology – Plant

Flooding enhances Ethylene entrapment and biosynthesis, followed by a decrease in ABA concentration and an increase in responsiveness to the hormone gibberellin (GA)

TLS Online TPP Program

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#Unit 6. System Physiology – Plant

The stomatal response to blue light is rapid and reversible, and it is localized in a single cell type, the guard cell


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#Unit 6. System Physiology – Plant

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#Unit 6. System Physiology – Plant

Guard cell volume depends on H+-ATPase. Orthovanadate, an inhibitor of the H+-ATPase,
inhibits this swelling

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#Unit 6. System Physiology – Plant

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#Unit 6. System Physiology – Plant

Changes in osmotic potential of guard cells required for stomatal opening in the morning are mediated by potassium and its counterions, whereas the afternoon changes are mediated by sucrose.

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