The logic for hotstart PCR is as follows, most DNA samples used as template will have some single-stranded DNA. 

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#Id: 6523

#Unit 7. System Physiology – Animal

Epinephrine and norepinephrine both act on α-and β-adrenergic receptors (adrenoceptors)

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#Id: 1424

#Unit 4. Cell Communication and Cell Signaling

The α subunit of each integrin is the primary determinant of its extracellular ligand specificity.

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#Id: 10386

#Unit 4. Cell Communication and Cell Signaling

Presentation of nonpeptide antigens derived from pathogens involves the nonclassical class I–like CD1 molecule

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#Id: 99

#Unit 5. Developmental Biology

In Conditional Specification

Prospective potency is greater than prospective fate.

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#Id: 4032

#Unit 7. System Physiology – Animal

Stimulation of the sympathetic cardiac nerves speeds the depolarization in SA node.