Allele-specific polymerase chain reaction (AS-PCR) is a technique based on allele-specific primers, which can be used to analyze single nucleotide polymorphism (SNP) effectively including the transition, transversion and insertion/deletion polymorphism and has been exploited in the study of diseases research, molecular diagnosis, and forensic biological evidence. 

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Five ways to splice an RNA

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Steric Hindrance and Combinations of Major and Minor Splice Sites are major reasons behind alternative splicing (Mutually Exclusive Splicing) 

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Alternative splicing is often associated with weak splice sites.

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Binding of SR proteins to ESEs promotes or stabilizes U1 binding to the 5’ splice site and U2AF binding to the 3’ splice site.

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The Nova and Fox families of RNA-binding proteins can promote or suppress splice site selection in a context-dependent fashion.

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#Unit 3. Fundamental Processes

Trans-splicing is rare in multicellular eukaryotes, it occurs as the primary mechanism to process precursor RNA into mature, translatable mRNAs in some organisms, such as trypanosomes and nematodes.