RACE can provide the sequence of an RNA transcript from a small known sequence within the transcript to the 5' end (5' RACE-PCR) or 3' end (3' RACE-PCR) of the RNA. 

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#Unit 2. Cellular Organization

In mammalian cells, Rab5 protein (early endosomes) and its effector protein known as EEA1 (early endosome antigen 1)

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#Unit 2. Cellular Organization

Effector proteins of Rab6 is Kinesin

Effector proteins of Rab27 is Type V Myosin

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#Unit 2. Cellular Organization

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#Unit 2. Cellular Organization

Sets of SNARE Proteins responsible for the eventual fusion of a vesicle with the target membrane:

Vesicle-specific membrane protein, v-SNARE

Target membrane contains t-SNARE

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#Unit 2. Cellular Organization

SNARE-mediated fusion occurs during exocytosis of secreted proteins. In this case, 

v-SNARE, are VAMP 

t-SNAREs are syntaxin & SNAP-25

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#Unit 2. Cellular Organization

SNARE complex is disassembled by ATP-dependent reaction mediated by cytosolic proteins, NSF & α-SNAP