RACE can provide the sequence of an RNA transcript from a small known sequence within the transcript to the 5' end (5' RACE-PCR) or 3' end (3' RACE-PCR) of the RNA. 

TLS Online TPP Program

#Id: 2617

#Unit 13. Methods in Biology

Type I restriction endonucleases cleave the DNA about 1000 bp away from the 5'-end of the sequence "TCA" located within the recognition site.

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#Id: 2618

#Unit 13. Methods in Biology

Type II restriction endonucleases are remarkably stable and induce cleavage either, in most cases, within or immediately outside their recognition sequences.

TLS Online TPP Program

#Id: 2619

#Unit 13. Methods in Biology

More than 350 different type II endonucleases with over 100 different recognition sequences are known.

TLS Online TPP Program

#Id: 2620

#Unit 13. Methods in Biology

Some restriction endonucleases and their recognition sites.

TLS Online TPP Program

#Id: 2621

#Unit 13. Methods in Biology

Examples of restriction endonuclease nomenclature.

TLS Online TPP Program

#Id: 2622

#Unit 13. Methods in Biology

Characteristics of the different types of endonucleases.