Primer extension Using primer extension one can locate the 59-end of a transcript by hybridizing an oligonucleotide primer to the RNA of interest, extending the primer with reverse transcriptase to the 5’-end of the transcript, and electrophoresing the reverse transcript to determine its size

TLS Online TPP Program

#Id: 10420

#Unit 4. Cell Communication and Cell Signaling

Heart of the EMT are two transcription factors, 

Snail and Twist

TLS Online TPP Program

#Id: 10421

#Unit 4. Cell Communication and Cell Signaling

Snail and Twist promote expression of genes involved in cell migration, loss of cell-cell adhesion, loss of cell polarity, and increase the production of proteases that digest the basement membrane

TLS Online TPP Program

#Id: 10422

#Unit 4. Cell Communication and Cell Signaling

TLS Online TPP Program

#Id: 10423

#Unit 4. Cell Communication and Cell Signaling

Tumor cells secrete a protein plasminogen activator that converts the serum protein plasminogen to the active protease plasmin.

TLS Online TPP Program

#Id: 10424

#Unit 4. Cell Communication and Cell Signaling

Noncoding RNAs, especially microRNAs, play a critical role in tumorigenesis:

Let-7 miRNAs

miR-15-a 

miR-16-1

miR-21

TLS Online TPP Program

#Id: 10425

#Unit 4. Cell Communication and Cell Signaling

Let-7 miRNAs repress the translation of Ras mRNA and also targets MYC