The media should contain all the essential constituents required for the growth of the microorganisms

Source of carbon

Source of nitrogen

Growth factors

Water

Antifoams

Trace elements

TLS Online TPP Program

#Id: 2272

#Unit 3. Fundamental Processes

The seven subunits of sigma subunits of E.Coli

TLS Online TPP Program

#Id: 2273

#Unit 3. Fundamental Processes

The lac promoter along with the bulk of other E. coli promoters, is recognized by RNA polymerase bearing the σ70 subunit.

TLS Online TPP Program

#Id: 2274

#Unit 3. Fundamental Processes

E. coli encodes several other s subunits that can replace σ 70 under certain circumstances.

a.σ 32 

b.σ 54

TLS Online TPP Program

#Id: 2275

#Unit 3. Fundamental Processes

The subunits of RNA Polymerases

TLS Online TPP Program

#Id: 2276

#Unit 3. Fundamental Processes

Template and transcript within the RNA polymerase elongating complex

TLS Online TPP Program

#Id: 2277

#Unit 3. Fundamental Processes

Core enzyme without the s subunit could not transcribe viral DNA, yet had no problems with highly nicked calf thymus DNA