#Id: 2726
Scorable reporter genes
The gene encoding green fluorescent protein (GFP) isolated from the jellyfish (Aequorea victoria) has emerged as a very useful scorable marker, and it does not require addition of any substrate, cofactor.
Gene uidA encodes the enzyme β-glucourinidase, which acts on the substrate X-gluc (5-Br-4 Cl-3-indolyl-β-D-glucuronide). The substrate is cleaved by the enzyme within plant cells (transformed/transgenic), and the product is converted into an insoluble blue precipitate at the site of enzyme activity.
#Unit 12. Applied Biology
#Transgenic animals and plants
#Part B Pointers
