The run-on assay can also be used to determine the effects of assay conditions on nuclear transcription.

TLS Online TPP Program

#Id: 8367

#Unit 2. Cellular Organization

Myosin II

Two heads and assembles into bipolar filaments

Small and non-processive

Function- Contraction

TLS Online TPP Program

#Id: 8368

#Unit 2. Cellular Organization

Myosin V

Two heads but does not assemble into filaments

Large and processive

Function- Organelle transport

TLS Online TPP Program

#Id: 8369

#Unit 2. Cellular Organization

The length of the myosin II neck domain determines the rate of movement.

TLS Online TPP Program

#Id: 8370

#Unit 2. Cellular Organization

In the absence of ATP, the myosin head is firmly attached to the actin filament. 

TLS Online TPP Program

#Id: 8371

#Unit 2. Cellular Organization

In the presence of ATP,  myosin head releases from the actin filament. 

TLS Online TPP Program

#Id: 8372

#Unit 2. Cellular Organization

Thick-filament regulation

Myosin-dependent mechanism regulate contraction in smooth muscle and non-muscle cells.