Linker and/or adapter molecules can be joined to the cut ends. Linkers are short, chemically synthesized, double-stranded oligonucleotides, which contain within them one or more restriction endonuclease sites, e.g., linker 5'CCGAATTCGG (only one strand of the linker is shown here) contains one EcoRI site. 

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#Unit 6. System Physiology – Plant

Cytokinin binds to the CRE1 receptors to initiate a response that culminates in the elevation of transcription of the type- BARRs/ type- A ARRs. 

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#Unit 6. System Physiology – Plant

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#Unit 6. System Physiology – Plant

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#Unit 6. System Physiology – Plant

The isopentenyltransferase gene (IPT) that encodes a rate limiting enzyme in cytokinin biosynthesis, under the control of a water-deficit responsive and maturation specific promoter PSARK was introduced into cotton and the performance of the PSARK::IPT transgenic cotton plants was analyzed in the greenhouse and growth chamber conditions. 

The data indicate that PSARK::IPT-transgenic cotton plants displayed delayed senescence under water deficit conditions in the greenhouse. 

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#Unit 6. System Physiology – Plant

PSARK::IPT transgenic cotton plants produced more root and shoot biomass, dropped fewer flowers, maintained higher chlorophyll content, and higher photosynthetic rates under reduced irrigation conditions in comparison to wild-type and segregated non-transgenic lines. 

Furthermore, PSARK::IPT-transgenic cotton plants grown in growth chamber condition also displayed greater drought tolerance.  These results indicate that water-deficit induced expression of an isopentenyltransferase gene in cotton could significantly improve drought tolerance.

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#Unit 6. System Physiology – Plant

KNOX genes

KNOX gene expression increases cytokinin levels in the shoot apical meristem by upregulating the cytokinin biosynthetic gene ISOPENTENYL TRANSFERASE7 (IPT7)