Linkers have the following two applications: creation of cohesive 'ends

on blunt~ended DNA fragments, and 

on fragments having unmatched or undefined sequences in their protruding ends. 

TLS Online TPP Program

#Id: 875

#Unit 13. Methods in Biology

HOT START PCR were devised to prevent non – specific amplification at low temperature.

TLS Online TPP Program

#Id: 876

#Unit 13. Methods in Biology

FRET is possible only when the distance between donor and acceptor are within 1 to 10 Å

TLS Online TPP Program

#Id: 877

#Unit 13. Methods in Biology

GFP chromophore comprises an oxidized form of the tripeptide –Ser65–Tyr66–Gly67–.

TLS Online TPP Program

#Id: 878

#Unit 13. Methods in Biology

The efficiency of FRET is inversely proportional to the sixth power of the distance between donor and acceptor.

TLS Online TPP Program

#Id: 879

#Unit 13. Methods in Biology

Short tandem repeats (STRs) are short repeated sequences of DNA (1–10 bp) that account for approximately  3% of the human genome.

TLS Online TPP Program

#Id: 880

#Unit 13. Methods in Biology

AAAAAAAAAAAAAAA (a 1-base repeat) and CACACACACACACACACACACA (a 2-basen repeating), CAGCAGCAGCAGCAGCAG (a 3 base repeating) are most common.