A gel mobility shift assay, is a method for detecting DNA–protein interaction relies on the fact that a small DNA has a much higher mobility in gel electrophoresis than the same DNA does when it is bound to a protein. 

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#Id: 5535

#Unit 7. System Physiology – Animal

The ascending limb, both the thin & the thick segments is virtually impermeable to water, a characteristic that is important for concentrating the urine.

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#Id: 5536

#Unit 7. System Physiology – Animal

The thin segment of the ascending limb has a much lower reabsorptive capacity than does the thick segment.

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#Id: 5537

#Unit 7. System Physiology – Animal

Thick segment of the ascending limb of loop of Henle, has thick epithelial cells that have high metabolic activity and are capable of active reabsorption of sodium, chloride, and potassium

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#Id: 5538

#Unit 7. System Physiology – Animal

In the thick segment of the ascending loop of Henle  reabsorption mechanism is load-dependent.

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#Id: 5539

#Unit 7. System Physiology – Animal

In the thick ascending loop,  Luminal membrane contains a three-ion cotransporter  known as Na+-K+-2Cl- cotransporter

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#Id: 5540

#Unit 7. System Physiology – Animal

Thick ascending limb is called the diluting segment because NaCl is reabsorbed but water is not reabsorbed along with it