UV radiation is used to cause DNA breakage. UV reacts quickly hence helps to catch momentary protein binding and can be used in vivo as it penetrates cell membranes easily. But gels from such assays are difficult to interpret.

TLS Online TPP Program

#Id: 3848

#Unit 5. Developmental Biology

For stalk and spore maturation, cAMP is produced by adenylate cyclase R (ACR)

TLS Online TPP Program

#Id: 3849

#Unit 5. Developmental Biology

ACR harbours seven transmembrane domains and is embedded in the nuclear membrane of prestalk cells.

TLS Online TPP Program

#Id: 3850

#Unit 5. Developmental Biology

ACG harbours two transmembrane domains & localizes to the membrane of the prespore vesicles.

TLS Online TPP Program

#Id: 3851

#Unit 5. Developmental Biology

ACG act as an osmosensor, so spores formed from the acg- cells will germinate irrespective of the osmotic conditions.

TLS Online TPP Program

#Id: 3852

#Unit 5. Developmental Biology

Adenylate cyclase B (ACB) is normally expressed in prestalk cells, but is upregulated in the prespore region of acg null structures.

TLS Online TPP Program

#Id: 3853

#Unit 5. Developmental Biology

In D. discoideum cAMP phosphodiesterase RegA plays a crucial role in regulating intracellular cAMP levels cells & level of PKA activity.