UV radiation is used to cause DNA breakage. UV reacts quickly hence helps to catch momentary protein binding and can be used in vivo as it penetrates cell membranes easily. But gels from such assays are difficult to interpret.

TLS Online TPP Program

#Id: 2661

#Unit 7. System Physiology – Animal

Thick ascending limb is called the diluting segment because NaCl is reabsorbed but water is not reabsorbed along with it.

TLS Online TPP Program

#Id: 2662

#Unit 7. System Physiology – Animal

The tubular fluid that leaves the thick ascending limb has a lower Na+ concentration and a lower osmolarity than blood.

TLS Online TPP Program

#Id: 2663

#Unit 7. System Physiology – Animal

Recycling of K+ 

Most, but not all, of the K+ that enters the cell through cotransporter leaves the cell across BM, A portion of the K+, diffuses back into the lumen.

TLS Online TPP Program

#Id: 2664

#Unit 7. System Physiology – Animal

The thick ascending limb of the loop of Henle is the site of action of the powerful “loop” diuretics.

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#Id: 2665

#Unit 7. System Physiology – Animal

“Loop” Diuretics are:

Furosemide

Ethacrynic acid

Bumetanide 

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#Id: 2666

#Unit 7. System Physiology – Animal

“Loop” diuretics, inhibits 1-sodium, 2-chloride, 1-potassium 

co-transporter.