The retention time tR for each analyte has two components

 i - dead time, tM. &

ii - adjusted retention time, t’R.

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#Id: 4729

#Unit 13. Methods in Biology

RNA gives absorbance at 595 nm in presence of Diphenylamine while gives directabsorbance at 540 nm.

TLS Online TPP Program

#Id: 4730

#Unit 13. Methods in Biology

The characteristic absorption parameter for the sample is the extinction coefficient a, yielding the correlation I= I 0 e -d .

TLS Online TPP Program

#Id: 4731

#Unit 13. Methods in Biology

The ratio T= I/I 0 is called transmission.

TLS Online TPP Program

#Id: 4732

#Unit 13. Methods in Biology

Algebraic transformation of the exponential correlation into an expression based on the decadic logarithm yields the law of Beer–Lambert:

where [d] = 1cm, [c] = 1mol^dm -3 , and [e] = 1dm^3mol -1 cm1 e is the molar absorption coefficient.

TLS Online TPP Program

#Id: 8064

#Unit 13. Methods in Biology

Circular dichroism (CD) spectroscopy technique is used to measures the absorption difference between left and right circularly polarized light

TLS Online TPP Program

#Id: 8065

#Unit 13. Methods in Biology