The retention time tR for each analyte has two components

 i - dead time, tM. &

ii - adjusted retention time, t’R.

TLS Online TPP Program

#Id: 4899

#Unit 13. Methods in Biology

The t-test is any statistical hypothesis test in which the test statistic follows a student’s t-distribution under the null hypothesis.

TLS Online TPP Program

#Id: 4900

#Unit 13. Methods in Biology

It can be used to determine if two sets of data are significantly different from each other and is most applied when the test statistic would follow a normal distribution if the value of a scaling term in the test statistic were known.

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#Id: 4902

#Unit 13. Methods in Biology

T-test uses means and standard deviations of two samples to make a comparison. The formula for T-test is given below:

TLS Online TPP Program

#Id: 4905

#Unit 13. Methods in Biology

Variance and Standard Deviation are the two important measurements in statistics.

TLS Online TPP Program

#Id: 4906

#Unit 13. Methods in Biology

Variance is a measure of how data points vary from the mean, whereas standard deviation is the measure of the distribution of statistical data.

TLS Online TPP Program

#Id: 4908

#Unit 13. Methods in Biology

According to layman’s words, the variance is a measure of how far a set of data are dispersed out from their mean or average value. It is denoted as ‘σ²’.