Retention factor (k) is simply the additional time that the analyte takes to elute from the column relative to an unretained or excluded analyte that does not interact with the stationary phase and which, by definition, has a k value of 0. Thus

TLS Online TPP Program

#Id: 746

#Unit 3. Fundamental Processes

Hydrolysis of pyrophosphate makes phosphodiester bond a highly favorable reaction with a DG of –7 kcal/mol, which corresponds to an equilibrium constant (Keq) of 105.

TLS Online TPP Program

#Id: 747

#Unit 3. Fundamental Processes

A:T and G:C base pairs have same geometry that’s why DNA polymerase has only one catalytic site for polymerization 

TLS Online TPP Program

#Id: 748

#Unit 3. Fundamental Processes

Incorrect base pairing leads to dramatically lower rates of nucleotide addition called as kinetic proofreading

TLS Online TPP Program

#Id: 749

#Unit 3. Fundamental Processes

A common method to monitor synthesis of new DNA is Incorporation Assays.

TLS Online TPP Program

#Id: 750

#Unit 3. Fundamental Processes

rNTPs are present at approximately 10-fold higher concentration in the cell, they are incorporated at a rate that is more than 1000-fold lower than dNTPs.

TLS Online TPP Program

#Id: 751

#Unit 3. Fundamental Processes

The palm domain is composed of a b sheet and contains the primary elements of the catalytic site.