Retention factor (k) is simply the additional time that the analyte takes to elute from the column relative to an unretained or excluded analyte that does not interact with the stationary phase and which, by definition, has a k value of 0. Thus

TLS Online TPP Program

#Id: 375

#Unit 2. Cellular Organization

Circular DNA molecules purified from both bacteria and eukaryotes are usually negatively supercoiled, having values of s of approximately –0.06.

TLS Online TPP Program

#Id: 376

#Unit 2. Cellular Organization

How to calculate linking number?

TLS Online TPP Program

#Id: 377

#Unit 2. Cellular Organization

For negatively supercoiled circle W < 0 

For a positively coiled superhelix W > 0.

 

TLS Online TPP Program

#Id: 378

#Unit 2. Cellular Organization

Topological states of covalently closed, circular (ccc) DNA. 

TLS Online TPP Program

#Id: 379

#Unit 2. Cellular Organization

A conserved region found in every core histone, called the histone-fold domain HFD.

TLS Online TPP Program

#Id: 380

#Unit 2. Cellular Organization

The histone-fold domain is composed of three a-helical regions separated by two short unstructured loops.