notSelectivity or separation factor, a, which can also be viewed as simply the relative retention ratio for the two analytes:

TLS Online TPP Program

#Id: 2994

#Unit 3. Fundamental Processes

Nick translation has been a major technique for introducing radioactively labeled nucleotides into DNA in vitro.

TLS Online TPP Program

#Id: 2996

#Unit 3. Fundamental Processes

The coupled 5'-3' synthetic/ 3'-5' exonucleolytic action is used most extensively for filling in short single-stranded regions in double-stranded DNA. These regions arise during lagging strand DNA replication.

TLS Online TPP Program

#Id: 2997

#Unit 3. Fundamental Processes

These Okazaki fragments are found in replicating DNA in both prokaryotes and eukaryotes.

TLS Online TPP Program

#Id: 2998

#Unit 3. Fundamental Processes

On the leading strand (sometimes called the forward strand) DNA synthesis can proceed continuously in the 5 ' to 3’ direction as the parental duplex is unwound.

TLS Online TPP Program

#Id: 3005

#Unit 3. Fundamental Processes

On the lagging strand a stretch of single-stranded parental DNA must beexposed, and then a segment is synthesized in the reverse direction (relative to fork movement).

TLS Online TPP Program

#Id: 3006

#Unit 3. Fundamental Processes

These Okazaki fragments are found in replicating DNA in both prokaryotes and eukaryotes.