In NPLC the most popular stationary phase is an alkylamine bonded to silica. The mobile phase is generally an organic solvent such as hexane, heptane, dichloromethane or ethyl acetate.

TLS Online TPP Program

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#Unit 1. Molecules and their Interaction Relevant to Biology

Van der Waals interaction is the weakest of all intermolecular attractions between molecules.

TLS Online TPP Program

#Id: 8249

#Unit 1. Molecules and their Interaction Relevant to Biology

The van der Waals interaction primarily depends on interatomic distance.

TLS Online TPP Program

#Id: 8250

#Unit 1. Molecules and their Interaction Relevant to Biology

Fundamental Behind VWI is DIPOLE

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#Unit 1. Molecules and their Interaction Relevant to Biology

Electrons are constantly are moving in an atom, so dipoles are probable of occurring.

A dipole is defined as molecules or atoms with equal and opposite electrical charges separated by a small distance.

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#Unit 1. Molecules and their Interaction Relevant to Biology

There are four major DIPOLES that decide VWI

1. spontaneous (or instantaneous) dipoles

2. Dipole-Dipole Interaction

3. Induced Dipoles

4. Spontaneous Dipole-Induced Dipole Interaction

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#Unit 1. Molecules and their Interaction Relevant to Biology

Spontaneous (or instantaneous) dipoles

When groups of electrons move to one end of the atom, it creates a dipole.