These histidine's are separated by using Nickel-chelate-nitrilotriacetate (Ni-NTA) chromatography column.

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#Id: 4743

#Unit 5. Developmental Biology

Cortical cytoplasm contains PAR-3, PAR-6 & PKC-3 Internal cytoplasm contains PAR-2 and PAR-1

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#Id: 4744

#Unit 5. Developmental Biology

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#Id: 4745

#Unit 5. Developmental Biology

PKC-3 restricts PAR-1 and PAR-2 to the internal cytoplasm by phosphorylating them.

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#Id: 4746

#Unit 5. Developmental Biology

Sperm microtubules contacts the cortical cytoplasm and initiates cytoplasmic movements that push the male pronucleus to the nearest end of the oblong oocyte.

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#Unit 5. Developmental Biology

Sperm microtubules protect PAR-2 protein from phosphorylation, allowing it to enter the cortex along with its binding partner, PAR-1.

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#Id: 4748

#Unit 5. Developmental Biology

Once PAR-1 is in the cortical cytoplasm, it phosphorylates PAR-3 causing PAR-3 and its binding partners PAR-6 and PKC-3 to leave the cortex.