Phosphorylated protein or amino acid are separated by using immobilized metal affinity chromatography ( IMAC), or immuno-affinity using antibodies against phosphorylated amino acids or peptides

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#Id: 7242

#Unit 5. Developmental Biology

Progress through their developmental program

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#Unit 4. Cell Communication and Cell Signaling

Ligand binding depends on: 

Multiple weak, noncovalent forces

Molecular complementarity

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#Id: 3998

#Unit 2. Cellular Organization

One approach is to measure the effect of nicking the DNA. Unconstrained supercoils are released by nicking, whereas constrained supercoils are unaffected. Nicking releases -50% of the overall supercoiling.

Another approach uses the crosslinking reagent psoralen, which binds more readily to DNA when it is under torsional tension. The reaction of psoralen with E. coli DNA in vivo corresponds to an average density of 1 negative super helical turn/200 bp (sigma = -0.05).

TLS Online TPP Program

#Id: 10353

#Unit 4. Cell Communication and Cell Signaling

TLS Online TPP Program

#Id: 8359

#Unit 2. Cellular Organization

In Golgi complex

Arp2/3-dependent assembly of actin occur by an NPF WHAMM participating in membrane traffic from the ER to golgi