RNA gives absorbance at 595 nm in presence of Diphenylamine while gives direct absorbance at 540 nm.

TLS Online TPP Program

#Id: 753

#Unit 3. Fundamental Processes

One metal ion generate a 3’O- that is primed for the nucleophilic attack of the a-phosphate of the incoming dNTP.

TLS Online TPP Program

#Id: 754

#Unit 3. Fundamental Processes

The second metal ion coordinates the negative charges of the b-phosphate and g-phosphate of the dNTP and stabilizes the pyrophosphate produced by joining the primer and the incoming nucleotide

TLS Online TPP Program

#Id: 755

#Unit 3. Fundamental Processes

DNA polymerase “grips” the template and the incoming nucleotide when a correct base pair is made via Arg, Lys and Tyr residue in its alpha helix

TLS Online TPP Program

#Id: 756

#Unit 3. Fundamental Processes

Base-pair geometry and the complementarity between the bases cannot reach the extraordinarily high levels of accuracy

TLS Online TPP Program

#Id: 757

#Unit 3. Fundamental Processes

Approximately one mistake in every 1010 bp added due to keto-enol tautomerism.

TLS Online TPP Program

#Id: 2882

#Unit 3. Fundamental Processes

Initiation at oriC requires the sequential assembly of a large protein complex on the membrane.