The absorption of UV light by nucleic acids arises from n ® p* and p ® p* transitions of the purine and pyrimidine bases that occur between 260nm and 275 nm.

TLS Online TPP Program

#Id: 4592

#Unit 13. Methods in Biology

2D Histogram

TLS Online TPP Program

#Id: 4593

#Unit 13. Methods in Biology

Flow cytometry is majorly used with fluorescence dyes and attached antibodies for cells and dyes.

TLS Online TPP Program

#Id: 4594

#Unit 13. Methods in Biology

Tritiated (3H) Thymidine uptake used to assess Cell Division based on radioactivity measurement by scintillation counting.

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#Id: 4595

#Unit 13. Methods in Biology

Tetrazolium compound MTT (3-(4,5-Dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide MTT Assay is calorimetric assay to detect cell division

TLS Online TPP Program

#Id: 4596

#Unit 13. Methods in Biology

Diphenyltetrazolium bromide, a yellow tetrazole is reduced by metabolically active cells to form insoluble, purple formazan dye crystals detected at 570nm

TLS Online TPP Program

#Id: 4597

#Unit 13. Methods in Biology

The more metabolically active cells that are present in the culture, the more formazan will be generated, and so this assay provides a readout of the number of live cells as a function of time.