DnaG primase is bound to the helicase complex and creates the replication forks

TLS Online TPP Program

#Id: 5926

#Unit 3. Fundamental Processes

The termination sequences are unidirectional; that is, they function in only one orientation. The ter site is recognized by a unidirectional contrahelicase

TLS Online TPP Program

#Id: 5927

#Unit 3. Fundamental Processes

In E. coli, two clusters of five ter sites each, including terK, -I, -E, -D, and -A on one side and terC, -B, -F, -G, and -H on the other, are located 100 kb on either side of this termination region.

Each set of ter sites is specific for one direction of fork movement

TLS Online TPP Program

#Id: 5991

#Unit 3. Fundamental Processes

Eukaryotic replication is similar in most aspects to bacterial replication. It is semiconservative, bidirectional, and semidiscontinuous.

TLS Online TPP Program

#Id: 5992

#Unit 3. Fundamental Processes

Eukaryotic replicons are 40 to 100 kb in length and individual replicons are activated at characteristic times during S phase.

TLS Online TPP Program

#Id: 5993

#Unit 3. Fundamental Processes

Replicons in euchromatin initiate before replicons in heterochromatin

TLS Online TPP Program

#Id: 5994

#Unit 3. Fundamental Processes

The rate of replication is 2000 bp/min, which is much slower than the 50,000 bp/min of bacterial replication fork movement, presumably because the chromosome is assembled into chromatin, not naked DNA.