Hda protein is recruited to the replication origin to convert DnaA-ATP to DnaA-ADP.

TLS Online TPP Program

#Id: 8607

#Unit 2. Cellular Organization

Cdk1 

=Cdc2 (vertebrates and fission yeast)

= Cdc28  (budding yeast)

TLS Online TPP Program

#Id: 8608

#Unit 2. Cellular Organization

There are three D cyclins in mammals:

cyclins D1, D2, and D3

TLS Online TPP Program

#Id: 8609

#Unit 2. Cellular Organization

In free CDK2, the A-loop blocks access of protein substrates to the γ phosphate of the bound ATP.

TLS Online TPP Program

#Id: 8610

#Unit 2. Cellular Organization

TLS Online TPP Program

#Id: 8611

#Unit 2. Cellular Organization

Nonphosphorylated CDK bound to one of its cyclin partners has minimal but detectable protein kinase activity in vitro, although it may be essentially inactive in vivo.

TLS Online TPP Program

#Id: 8612

#Unit 2. Cellular Organization

Phosphorylation of a critical threonine residue in the activation loop, Thr-160, allowing substrates to access the active site of the CDK