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TLS Online TPP Program

#Id: 3043

The gap is filled by DNA polymerase I; pol A mutants fail to join their Okazaki fragments properly. 

#Unit 3. Fundamental Processes #Unit of replication and enzymes involved #Part B Pointers
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TLS Online TPP Program

#Id: 8771

#Unit 1. Molecules and their Interaction Relevant to Biology
TLS Online TPP Program

#Id: 8772

#Unit 1. Molecules and their Interaction Relevant to Biology

van’t Hoff plot is used measure rate of unfolding of proteins
TLS Online TPP Program

#Id: 8773

#Unit 1. Molecules and their Interaction Relevant to Biology

Techniques for Measuring Stability

Absorbance (e.g. Trp, Tyr) 

Fluorescence (Trp)-difference in emission max & intensity.

CD (far or near UV) - (2o or 3o) 

NMR 

DSC (calorimetry) 

Urea gradient gels - difference in the migrating rates between F and U.

Catalytic activity 

Chromophoric or fluorophoric probes/FRET 
TLS Online TPP Program

#Id: 8774

#Unit 1. Molecules and their Interaction Relevant to Biology

The Cp heat capacity of a defined system is the amount of heat (usually expressed in calories, kilocalories, or joules) needed to raise the system's temperature by one degree (usually expressed in Celsius or Kelvin).
TLS Online TPP Program

#Id: 8775

#Unit 1. Molecules and their Interaction Relevant to Biology

Thermal stability of protein can be calculated
TLS Online TPP Program

#Id: 8776

#Unit 1. Molecules and their Interaction Relevant to Biology