Nurturing Life Sciences
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#Unit 13. Methods in Biology
Flow cytometry is based on light scattering and fluorescence
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Forward light scattering detect shape and side scattering detects granularity and complexity of the cell
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Hydrodynamically focused stream separate cells in single file
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2D Histogram
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Flow cytometry is majorly used with fluorescence dyes and attached antibodies for cells and dyes.
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Tritiated (3H) Thymidine uptake used to assess Cell Division based on radioactivity measurement by scintillation counting.