Amplification (i.e., abnormal DNA replication) of a DNA segment including a proto-oncogene, leading to overproduction of the encoded protein.

TLS Online TPP Program

#Id: 8547

#Unit 1. Molecules and their Interaction Relevant to Biology

The irreversible inhibitors cannot be removed by simple physical techniques such as dialysis, is to reduce the amount of enzyme available for reaction.

TLS Online TPP Program

#Id: 8548

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 8549

#Unit 1. Molecules and their Interaction Relevant to Biology

The irreversible inhibition involves reactions with a functional group, such as hydroxyl or sulphydryl, or with a metal atom in the active site or a distinct allosteric site.

TLS Online TPP Program

#Id: 8550

#Unit 1. Molecules and their Interaction Relevant to Biology

diisopropylphosphofluoridate, reacts with a serine OH group in the active site of esterases such as acetylcholinesterase/chymotrypsin, whilst the organomercury compound p-hydroxymercuribenzoate reacts with a cysteine group, in both cases resulting in covalent bond formation and enzyme inhibition.

TLS Online TPP Program

#Id: 8551

#Unit 1. Molecules and their Interaction Relevant to Biology

di-isopropylphosphofluoridate (DFP), phenylmethyl sulphonylfluoride

(PMSF) and tosylphenylalanyl-chloromethylketone (TPCK) (all serine protease inhibitors); 

iodoacetate and cystatin (thiol protease inhibitors); 

pepstatin (aspartic protease inhibitor); 

EDTA and 1,10-phenanthroline (metalloprotease inhibitors); and amastatin and bestatin (exopeptidase inhibitors).

TLS Online TPP Program

#Id: 8552

#Unit 1. Molecules and their Interaction Relevant to Biology