Pyruvate kinase acts in the nucleus to regulate the transcription of genes that respond to thyroid hormone. Glyceraldehyde 3-phosphate dehydrogenase moonlights both as uracil DNA glycosylase, effecting the repair of damaged DNA, and as a regulator of histone H2B transcription. 

TLS Online TPP Program

#Id: 8405

#Unit 2. Cellular Organization

Muscle contraction depends on two processes that consume enormous amounts of ATP: 

1. Filament sliding, driven by the ATPase of the myosin

2. Ca2+ pumping, driven by the Ca2+ ATPase

TLS Online TPP Program

#Id: 8406

#Unit 2. Cellular Organization

Vertebrate skeletal muscle contraction, also depends on set of specialized accessory proteins that are closely associated with the actin thin filaments:

a) Tropomyosin

b) Troponin

TLS Online TPP Program

#Id: 8408

#Unit 2. Cellular Organization

Tropomyosin (TM) is a ropelike molecule, about 40 nm in length, binds to 7 actin subunits in an actin filament.

TLS Online TPP Program

#Id: 8409

#Unit 2. Cellular Organization

Troponin, a complex of three polypeptides, 

TN-T (tropomyosin-binding)

TN-I  (inhibitory)

TN-C (Ca2+-binding)

TLS Online TPP Program

#Id: 8410

#Unit 2. Cellular Organization

TN-C is the calcium-binding subunit, controls the

position of TM on the surface of an actin filament through the TN-I and TN-T subunits

TLS Online TPP Program

#Id: 8411

#Unit 2. Cellular Organization

In the absence of Ca2+, muscle is relaxed

TM blocks myosin’s interaction with F-actin