The sum of the number of unique genes and the number of gene families is an estimate of the number of types of genes.

The minimum size of the proteome can be estimatedfrom the number of types of genes.

TLS Online TPP Program

#Id: 1846

#Unit 3. Fundamental Processes

Molecular genetic techniques are used to make a cDNA encoding a recombinant protein to which is fused a short sequence of amino acids called an epitope tagging.

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#Id: 1847

#Unit 3. Fundamental Processes

Two commonly used epitope tags are FLAG, which encodes the amino acid sequence DYKDDDDK (single-letter amino acid code), and myc, which encodes the sequence EQKLISEEDL.

TLS Online TPP Program

#Id: 1848

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 1849

#Unit 3. Fundamental Processes

Fluorochrome-coupled monoclonal antibodies to the FLAG or myc epitopes can then be used to detect the recombinant protein in the cell.

Alexa- 488-labeled antibody -emit green light 

Alexa-568-labeled antibody- emit red light

TLS Online TPP Program

#Id: 1850

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 1851

#Unit 3. Fundamental Processes