To check the cell viability in cell suspension, trypan blue dye is used. This dye is excluded by viable cells but taken up by dead cells (nonviable cells).

TLS Online TPP Program

#Id: 397

#Unit 2. Cellular Organization

Nucleosomal DNA wraps around the histone protein 1.65 times, so create a writhe of –1.65 and so DLk should be -1.65. But when the change in linking number associated with each nucleosome was measured, the number was lower than this, approximately –1.2 for each nucleosome added. This discrepancy is referred to as the “Nucleosome Linking Number paradox,”

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#Id: 7394

#Unit 5. Developmental Biology

TLS Online TPP Program

#Id: 3856

#Unit 5. Developmental Biology

Dictyostelium can utilize one of three survival strategies when food starvation-

(1) Microcyst formation, favoured in dark & wet conditions, or ammonia levels are high.
(2) Zygote formation, favoured in dark & wet conditions, & triggered by ethylene produced by the cells.
(3) Cell aggregation & differentiation

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#Id: 3674

#Unit 3. Fundamental Processes

Many prokaryotic ORFs contain a short sequence upstream (on the 5’side) of the start codon called the ribosome binding site (RBS). This element is also referred to as a Shine –Dalgarno sequence.

TLS Online TPP Program

#Id: 1613

#Unit 4. Cell Communication and Cell Signaling

NF-₭B is key for inducing many innate and inflammatory genes, including those encoding defensins; enzymes such as iNOS.