Carboxyfluorescein succinimidyl ester (CFSE) is more correctly named carboxyfluorescein diacetate succinimidyl ester (CFDASE) very commonly used to detect state of cell

#Id: 2399

#Unit 3. Fundamental Processes

There are two classes of snoRNPs involved in rRNA processing in nucleolus. 

The box H/ACA snoRNPs are involved in pseudouridylylation, and box C/D snoRNPs function in 2-O-methylations.

#Id: 2400

#Unit 3. Fundamental Processes

Sigma Region 3/4 linker mimics RNA

#Id: 2401

#Unit 3. Fundamental Processes

Region ¾ linker of s lies in the middle of the RNA exit channel in the open complex

#Id: 2402

#Unit 3. Fundamental Processes

TF11S displays little similarity in sequence or structure to the Gre factors, but it binds to the same part of the enzyme, the RNA polymerase secondary channel (pore). 

#Id: 2403

#Unit 3. Fundamental Processes

The cleavage activity is intrinsic to RNA polymerase itself, it is stimulated greatly by accessory factors that are ubiquitous in the three biological kingdoms. There are two such factors in E. coli, GreA and GreB, and eukaryotic RNA polymerase II uses TFIIS for the same purpose. 

#Id: 2404

#Unit 3. Fundamental Processes

The Gre factors/TF11S enable the polymerase to cleave a few ribonucleotides from the 3' terminus of the RNA product, thereby allowing the catalytic site of RNA polymerase to be realigned with the 3' -OH.