Antibody and soluble antigen interacting in aqueous solution form a lattice that eventually develops into a visible precipitate. Antibodies that aggregate soluble antigens are called precipitins

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#Unit 13. Methods in Biology

Phenomenon of Electroendosmosis is largely applicable in Free Solution & Capillary.

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#Unit 13. Methods in Biology

Electrophoretic mobility and Electroendosmosis force combinedly functions in Capillary zone Electrophoresis

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#Unit 13. Methods in Biology

In electrophoretic separation the phenomenon of electroendosmosis (also known as electro-osmotic flow) which is due to the presence of charged groups on the surface of the support medium.

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#Unit 13. Methods in Biology

Three basically different electrophoretic separation methods are performed in practice nowadays

 Electrophoresis, sometimes called Zone Electrophoresis (ZE) GE

 Isotachophoresis (ITP) SDS PAGE

Isoelectric focusing (IEF).

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#Unit 13. Methods in Biology

In ZE a homogeneous buffer system is used over the whole separation time and range so as to ensure a constant pH value.

#Id: 2474

#Unit 13. Methods in Biology

In ITP, the separation is carried out in a discontinuous buffer system. The ionized sample migrates between a leading electrolyte with a high mobility and a terminating – sometimes called trailing – ion with a low mobility, all of them migrating with the same speed.