Flow cytometry or FACS is the sine qua non (without which, nothing) of the modern immunologist’s toolbox.

Flow cytometry is based on light scattering and fluorescence 

Forward light scattering detect shape and side scattering detects granularity and complexity of the cell

TLS Online TPP Program

#Id: 3224

#Unit 3. Fundamental Processes

Xeroderma pigmentosum (XP) is a human disease caused by mutations in any one of several nucleotide excision repair genes.

TLS Online TPP Program

#Id: 3226

#Unit 3. Fundamental Processes

Numerous proteins, including XP products and the transcription factor TFnH, are involved in eukaryotic nucleotide excision repair. 

TLS Online TPP Program

#Id: 3228

#Unit 3. Fundamental Processes

Global genome repair recognizes damage anywhere in the genome.

TLS Online TPP Program

#Id: 3230

#Unit 3. Fundamental Processes

Transcriptionally active genes are preferentially repaired via transcription-coupled repair.

TLS Online TPP Program

#Id: 3233

#Unit 3. Fundamental Processes

Global genome repair and transcription-coupled repair differ in their mechanisms of damage recognition (XPC vs. RNA polymerase II).

TLS Online TPP Program

#Id: 3236

#Unit 3. Fundamental Processes

transitions, which are pyrimidine-to-pyrimidine and purine-to-purine substitutions, such as T to C and A to G;