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The more metabolically active cells that are present in the culture, the more formazan will be generated, and so this assay provides a readout of the number of live cells as a function of time





#Unit 13. Methods in Biology #Immunotechniques #Part B Pointers
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TLS Online TPP Program

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#Unit 5. Developmental Biology

Unpaired protein activates the JAK-STAT pathway in the adjacent germ stem cells to specify their self-renewal

TLS Online TPP Program

#Id: 941

#Unit 5. Developmental Biology

 The mode of fertilization in sea urchin being external, the egg and spermatozoa are released into the sea water.

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#Id: 942

#Unit 5. Developmental Biology

Two major mechanisms during fertilization in sea urchin:
Species-specific sperm attraction 
Species-specific sperm activation

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#Id: 943

#Unit 5. Developmental Biology

Chemotaxis 
sperms are attracted toward eggs of their species by following a gradient of a chemical secreted by the egg.

TLS Online TPP Program

#Id: 944

#Unit 5. Developmental Biology

In Sea urchin,
Sperm-activating peptides provide direction to sperm during chemotaxis.

TLS Online TPP Program

#Id: 945

#Unit 5. Developmental Biology

SAP in the egg jelly of the sea urchin Arbacia punctulata 
Resact (14-amino acid chemotactic peptide)

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