bromodeoxyuridine (BrdU) is rapidly phosphorylated and incorporated in its place into newly synthesized DNA hence BrdU is used to detect replicating cells

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#Id: 2600

#Unit 13. Methods in Biology

Touch down PCR are derived to enhance primer binding in the annealing step.

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#Id: 2601

#Unit 13. Methods in Biology

A modification of overlap extension PCR may also be used to construct directed mutations; this is termed megaprimer PCR .

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#Id: 2602

#Unit 13. Methods in Biology

Long primers forms most probably dimers, so taq pol amplifying this (in low temp) also.

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#Id: 2603

#Unit 13. Methods in Biology

The SNP mutation responsible for sickle-cell disease changes amino acid 6 in haemoglobin β from glutamic acid (E) to valine (V). 

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#Id: 2604

#Unit 13. Methods in Biology

The sequence of the PCR product from a heterozygous carrier shows both the normal (HbβA) and mutant (HbβS) nucleotides at the position of the substitution SSR genotyping by PCR product electrophoresis.

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#Id: 2605

#Unit 13. Methods in Biology

Many different alleles are present in a population, but each person has only two alleles of any given SSR locus.