Common colorimetric and UV absorption assays

TLS Online TPP Program

#Id: 7450

#Unit 5. Developmental Biology

ACG harbours two transmembrane domains & localizes to the membrane of the prespore vesicles.

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#Id: 7451

#Unit 5. Developmental Biology

During spore formation, when the prespore vesicles fuse with the plasma membrane, ACG localizes to the spore surface.

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#Id: 7452

#Unit 5. Developmental Biology

ACG act as an osmosensor, so spores formed from the acg- cells will germinate irrespective of the osmotic conditions.

TLS Online TPP Program

#Id: 7453

#Unit 5. Developmental Biology

Adenylate cyclase B (ACB) is normally expressed in prestalk cells, but is upregulated in the prespore region of acg null structures. 

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#Id: 7454

#Unit 5. Developmental Biology

In D. discoideum cAMP phosphodiesterase RegA plays a crucial role in regulating intracellular cAMP levels cells & level of PKA activity.

TLS Online TPP Program

#Id: 7455

#Unit 5. Developmental Biology

RegA is activated by phosphorylation of an aspartate in its response regulator domain.