During first cleavage, the metaphase plate is closer to the posterior, & fertilized egg is divided into two cells:

one having the anterior PARs (PAR-6 and PAR-3)

one having the posterior PARs (PAR-2 and PAR-1)

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#Id: 4645

#Unit 13. Methods in Biology

Tritiated (3H) Thymidine uptake used to assess Cell Division based on radioactivity measurement by scintillation counting. 

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#Id: 4646

#Unit 13. Methods in Biology

Diphenyltetrazolium bromide, a yellow tetrazole is reduced by metabolically active cells to form insoluble, purple formazan dye crystals detected at 570nm

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#Id: 4647

#Unit 13. Methods in Biology

The more metabolically active cells that are present in the culture, the more formazan will be generated, and so this assay provides a readout of the number of live cells as a function of time

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#Unit 13. Methods in Biology

 The MTT assay can measure cell proliferation or cell death.

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#Unit 13. Methods in Biology

bromodeoxyuridine (BrdU) is rapidly phosphorylated and incorporated in its place into newly synthesized DNA hence BrdU is used to detect replicating cells

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#Unit 13. Methods in Biology

BrdU can CAUSE light induced cell death.