Probability distribution yields the possible outcomes for any random event. It is also defined based on the underlying sample space as a set of possible outcomes of any random experiment.

TLS Online TPP Program

#Id: 3972

#Unit 2. Cellular Organization

TLS Online TPP Program

#Id: 3974

#Unit 2. Cellular Organization

E. coli has at least four individual topoisomerases (I through IV). Those of type I (topoisomerases I and III) generally, relax DNA by removing negative supercoils (increasing Lk).

TLS Online TPP Program

#Id: 3976

#Unit 2. Cellular Organization

A bacterial type II enzyme, called either topoisomerase II or DNA gyrase, can introduce negative supercoils (decrease Lk). It uses the energy of ATP to accomplish this.

To alter DNA linking number, type II topoisomerases cleave both strands of a DNA molecule and pass another duplex through the break.

TLS Online TPP Program

#Id: 3977

#Unit 2. Cellular Organization

The degree of supercoiling of bacterial DNA is maintained by regulation of the net activity of topoisomerases, I and II.

TLS Online TPP Program

#Id: 3980

#Unit 2. Cellular Organization

Eukaryotic cells also have type I and type II topoisomerases. The type I enzymes are topoisomerases I and III;

The eukaryotic type II topoisomerases cannot underwind DNA (introduce negative supercoils), but they can relax both positive and negative supercoils.

TLS Online TPP Program

#Id: 3981

#Unit 2. Cellular Organization

The quinolone antibiotics, inhibits bacterial DNA gyrase and topoisomerase IV, as ciprofloxacin (Cipro). The quinolones act by blocking the last step of the topoisomerase reaction, the resealing of the DNA strand breaks. Ciprofloxacin is a wide-spectrum antibiotic.