Nurturing Life Sciences
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#Unit 13. Methods in Biology
CCG and CGG are the rarest trinucleotides in most A+T-rich bacterial genomes and CTAG is the rarest tetranucleotide in G+C-rich bacterial genomes.
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Recognize the same sequence and cut at exactly the same position, these enzymes are called isoschizomers.
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Isoschizomers are applicable in recognizing methylation sites genomes.
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Two REs recognize the same DNA sequence motif but cleave at different positions, then they are called neoschizomers
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Process of replication in which gap is created by DNase I, filled by klenow fragment and with radioactive dNTPs called as Nick Translation