TLS Online TPP Program

#Id: 5335


Atrial systole marks the end of diastole, at which point ventricular volume is equal to end-diastolic volume.




#Unit 7. System Physiology – Animal #Cardiac cycle, heart as a pump #Part B Pointers
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TLS Online TPP Program

#Id: 2471

#Unit 13. Methods in Biology

In electrophoretic separation the phenomenon of electroendosmosis (also known as electro-osmotic flow) which is due to the presence of charged groups on the surface of the support medium.




TLS Online TPP Program

#Id: 2472

#Unit 13. Methods in Biology

Three basically different electrophoretic separation methods are performed in practice nowadays


 Electrophoresis, sometimes called Zone Electrophoresis (ZE) GE


 Isotachophoresis (ITP) SDS PAGE


Isoelectric focusing (IEF).


TLS Online TPP Program

#Id: 2473

#Unit 13. Methods in Biology

In ZE a homogeneous buffer system is used over the whole separation time and range so as to ensure a constant pH value.


TLS Online TPP Program

#Id: 2474

#Unit 13. Methods in Biology

In ITP, the separation is carried out in a discontinuous buffer system. The ionized sample migrates between a leading electrolyte with a high mobility and a terminating – sometimes called trailing – ion with a low mobility, all of them migrating with the same speed.




TLS Online TPP Program

#Id: 2475

#Unit 13. Methods in Biology

IEF takes place in a pH gradient and can only be used for amphoteric substances such as peptides and proteins. The charged molecules move toward the anode or the cathode until they reach a position in the pH gradient where their net charges are zero. This pH value is the “isoelectric point” (pI) of the substance.


TLS Online TPP Program

#Id: 2476

#Unit 13. Methods in Biology

Agarose is one of the components of agar, which is a mixture of polysaccharides isolated from certain seaweeds and usually used at concentrations of between 1% and 3% in labs.