Nurturing Life Sciences
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#Unit 12. Applied Biology
Continuous cell lines are the cell lines that has gained the ability for infinite growth after transformation, which can occur spontaneously or can be chemically or virally induced.
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Hayflick limit of animal cell culture
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Most cells are anchorage dependent and must be cultured while attached to a solid or semi-solid substrate (adherent or monolayer culture), while others can be grown floating in the culture medium (suspension culture).
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The culture medium provides the necessary nutrients, growth factors, and hormones for cell growth, as well as regulating the pH and the osmotic pressure of the culture.