The average number of nucleotides added before a polymerase dissociates defines its processivity. Nucleotides added per unit time dissociates defines its polymerisation

The 3’->5’ exonuclease activity removes the mispaired nucleotide, and the polymerase begins again known as proofreading

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#Unit 3. Fundamental Processes

An arrested RNA polymerase can restart transcription by cleaving the RNA transcript to generate a new 3' end.

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#Unit 3. Fundamental Processes

RNA polymerase performs two proofreading functions on that growing transcript

pyrophosphorolytic editing

hydrolytic editing

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#Unit 3. Fundamental Processes

In the Pyrophosphorolytic editing, the enzyme uses its active site, in a simple back-reaction, to catalyze the removal of an incorrectly inserted ribonucleotide, by reincorporation of PPi.

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#Unit 3. Fundamental Processes

Hydrolytic editing, is the polymerase backtracks by one or more nucleotides and cleaves the RNA product, removing the error-containing sequence.

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#Unit 3. Fundamental Processes

Hydrolytic editing is stimulated by Gre factors, which both enhance hydrolytic editing function and serve as elongation stimulating factors.

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#Unit 3. Fundamental Processes

The Nus proteins—joins polymerase in the elongation phase and promotes.