DNA polymerase E is a highly processive enzyme that continuously synthesizes the leading strand. Its processivity results from its interaction with RFC clamp loader and trimeric PCNA processivity clamp

TLS Online TPP Program

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#Unit 3. Fundamental Processes

The Nus proteins—joins polymerase in the elongation phase and promotes.

TLS Online TPP Program

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#Unit 3. Fundamental Processes

Both polymerase removal and repair enzyme recruitment are performed by a single protein called TRCF.

TLS Online TPP Program

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#Unit 3. Fundamental Processes

TRCF has an ATPase activity.

TLS Online TPP Program

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#Unit 3. Fundamental Processes

TRCF binds to double-stranded DNA upstream of the polymerase and uses the ATPase motor to translocate along the DNA until it encounters the stalled RNA polymerase.

TLS Online TPP Program

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#Unit 3. Fundamental Processes

In bacteria, terminators come in two types: Rho-dependent and Rho-independent. 

TLS Online TPP Program

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#Unit 3. Fundamental Processes

Rho-independent terminators, also called intrinsic terminators because they need no other factors to work, consist of two sequence elements: a short-inverted repeat (of 20 nucleotides) followed by a stretch of about eight A:T base pairs.