The deamination generates a G-T/C-T mismatch pair, MutL and MutS remove T from both G-T and C-T mismatches.

TLS Online TPP Program

#Id: 2892

#Unit 3. Fundamental Processes

DnaA is an ATP-binding protein. The first stage in initiation is binding of the DnaA-ATP 

protein complex to the fully methylated oriC sequence.

TLS Online TPP Program

#Id: 2893

#Unit 3. Fundamental Processes

DnaA is in the active form only when bound to ATP.

TLS Online TPP Program

#Id: 2894

#Unit 3. Fundamental Processes

Dna A has intrinsic ATPase activity that hydrolyzes ATP to ADP and thus inactivates itself when the initiation stage ends.

TLS Online TPP Program

#Id: 2895

#Unit 3. Fundamental Processes

This ATPase activity of Dna A is stimulated by membrane phospholipids and single-stranded DNA.

TLS Online TPP Program

#Id: 2897

#Unit 3. Fundamental Processes

The origin of the replication region remains attached to the membrane for about one-third of the cell cycle as another part of the mechanism to prevent reinitiation.

TLS Online TPP Program

#Id: 2899

#Unit 3. Fundamental Processes

Opening oriC involves action at two types of sequence in the origin: 9-bp and 13 -bp repeats.