Glycosylase action is followed by the endonuclease APE1, which cleaves the polynucleotide chain on the 5’ side. This in turn attracts a replication complex including the DNA polymerase d/E and ancillary components, which performs a short synthesis reaction extending for two to 10 nucleotides. The displaced material is removed by the endonuclease FEN1.

TLS Online TPP Program

#Id: 818

#Unit 3. Fundamental Processes

Sigma factor Region 2.3 is responsible for melting the DNA at -10 element.

TLS Online TPP Program

#Id: 1841

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 1842

#Unit 3. Fundamental Processes

Eukaryotic nuclei contain RNA polymerase I, RNA polymerase II, and RNA polymerase III can be separated by using DEAE-Sephadex ion-exchange chromatography

TLS Online TPP Program

#Id: 1843

#Unit 3. Fundamental Processes

The α-amanitin binding site is beneath a “bridge helix” extending across the cleft between the two largest pol II subunits, Rpb1 and Rpb2

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#Id: 1844

#Unit 3. Fundamental Processes

Rpo I =14 subunits

Rpo II = 12 subunits

Rpo III = 17 subunits

There are five common subunits

Rpb5, Rpb6, Rpb8, Rpb10, Rpb12

TLS Online TPP Program

#Id: 1845

#Unit 3. Fundamental Processes

RNA Polymerase can also separated by using epitope tagging. Normally RPB3 is used to create epitope tag.