These vectors are advantageous in that they can deliver many genome copies per host cell and so typically are associated with high expression. 

TLS Online TPP Program

#Id: 7097

#Unit 3. Fundamental Processes

In bacteria and mitochondria, the formyl residue on the initiator methionine is removed from the protein by a specific deformylase enzyme to generate a normal NH2 terminus.

TLS Online TPP Program

#Id: 7098

#Unit 3. Fundamental Processes

In about half the polypeptides, the methionine at the terminus is removed by an aminopeptidase, which creates a new terminus from R2 (originally the second amino acid incorporated into the chain). When both steps are necessary, they occur sequentially. The removal reaction(s) occur rather rapidly when the nascent polypeptide chain has reached a length of 15 amino acids.

TLS Online TPP Program

#Id: 7099

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 6452

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 6453

#Unit 3. Fundamental Processes

Liquid chromatography separates and identifies the three eukaryotic RNA polymerases, each with its own sensitivity to 𝛂-amanitin.

TLS Online TPP Program

#Id: 6454

#Unit 3. Fundamental Processes

The α-amanitin binding site is beneath a “bridge helix” extending across the cleft between the two largest pol II subunits, Rpb1 and Rpb2

a DNA template, whereas in eukaryotes, transcription takes place on a chromatin template.