Cytosine is deaminated to Uracil.

TLS Online TPP Program

#Id: 2493

#Unit 13. Methods in Biology

The band-sharpening effect relies on the fact that negatively charged glycinate ions (in the electrophoresis buffer) have a lower electrophoretic mobility than do the protein–SDS complexes, which, in turn, have lower mobility than the chloride ions (Cl − ) of the loading buffer and the stacking gel buffer. 

[Cl−] > [protein–SDS] > [glycinate].

TLS Online TPP Program

#Id: 2887

#Unit 3. Fundamental Processes

short region of A-T -rich DNA is melted.

TLS Online TPP Program

#Id: 875

#Unit 13. Methods in Biology

HOT START PCR were devised to prevent non – specific amplification at low temperature.

TLS Online TPP Program

#Id: 6708

#Unit 3. Fundamental Processes

Conversion of specific uridine residues to pseudouridine and the addition of methyl groups to the 2′-hydroxyl groups

of specific riboses, that are important for the proper assembly and function of snRNPs in pre-mRNA splicing. 

These modifications occur in Cajal bodies, where they are directed by a class of snoRNA-like guide RNA molecules called scaRNAs (small Cajal body–associated RNAs)

TLS Online TPP Program

#Id: 7321

#Unit 12. Applied Biology

Phytovolatilization

Plants remove inorganic contaminants· either by contaminant volatilization or by metal accumulation.

Volatalization is useful for the removal of mercury. A bacterial mercuric ion reductase has been transferred to Arabidopsis thaliana; this transgenic plant absorbs mercury, and reduces it to volatile Hg0, which volatilizes from the leaf surface.