TBP causes the minor groove to be widened to an almost flat conformation; it also bends the DNA by an angle of 80. The interaction between TBP and DNA involves only a limited number of hydrogen bonds between the protein and the edges of the base pairs in the minor groove.  Instead, much of the specificity is imposed by two pairs of phenylalanine side chains that intercalate between the base pairs at either end of the recognition sequence and drive the strong bend in the DNA.

TLS Online TPP Program

#Id: 4051

#Unit 2. Cellular Organization

The centromeres of all four chromosomes of D. melanogaster aggregate to form a chromocenter that consists largely of heterochromatin.

TLS Online TPP Program

#Id: 4052

#Unit 2. Cellular Organization

Each is produced by the successive replications of a synapsed diploid pair of chromosomes. The replicas do not separate, but instead remain attached to each other in their extended state, a process known as endoreduplication.

At the start of the process, each synapsed pair has a DNA content of 2C (where C represents the DNA content of the individual chromosome). This amount then doubles up to nine times, at its maximum giving a content of 1024C. The number of doublings is different in the various tissues of the D. melanogaster larva.

TLS Online TPP Program

#Id: 6359

#Unit 2. Cellular Organization

Operon consist

Physiology/Mechanism

Molecular biology/Biochemistry of component

Effect of mutations

TLS Online TPP Program

#Id: 6360

#Unit 2. Cellular Organization

Repressors impede access of RNA polymerase to the promoter and Activators enhance the RNA polymerase–promoter interaction.

TLS Online TPP Program

#Id: 6361

#Unit 2. Cellular Organization

Repressors bind to specific sites on the DNA. In bacterial cells, such binding sites, called operators

Regulation by means of a repressor protein that blocks transcription is referred to as negative regulation. Activators provide a molecular counterpoint to repressors; they bind to DNA and enhance the activity of RNA polymerase at a promoter; this is positive regulation.Repressor binding to DNA is regulated by a molecular signal called effector

TLS Online TPP Program

#Id: 6362

#Unit 2. Cellular Organization

Activators that stimulate this kind of promoter work by triggering a conformational change in either RNA polymerase or DNA; that is, they interact with the stable closed complex and induce a conformational change that causes transition to the open complex. This mechanism is an example of allostery.