TLS Online TPP Program

#Id: 8770

#Unit 1. Molecules and their Interaction Relevant to Biology

                                                                                                                                                                                                                                                                      

TLS Online TPP Program

#Id: 8771

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 8772

#Unit 1. Molecules and their Interaction Relevant to Biology

van’t Hoff plot is used measure rate of unfolding of proteins

TLS Online TPP Program

#Id: 8773

#Unit 1. Molecules and their Interaction Relevant to Biology

Techniques for Measuring Stability

Absorbance (e.g. Trp, Tyr) 

Fluorescence (Trp)-difference in emission max & intensity.

CD (far or near UV) - (2o or 3o) 

NMR 

DSC (calorimetry) 

Urea gradient gels - difference in the migrating rates between F and U.

Catalytic activity 

Chromophoric or fluorophoric probes/FRET 

TLS Online TPP Program

#Id: 8774

#Unit 1. Molecules and their Interaction Relevant to Biology

The Cp heat capacity of a defined system is the amount of heat (usually expressed in calories, kilocalories, or joules) needed to raise the system's temperature by one degree (usually expressed in Celsius or Kelvin).

TLS Online TPP Program

#Id: 8775

#Unit 1. Molecules and their Interaction Relevant to Biology

Thermal stability of protein can be calculated